Protein: Autophagy-related protein 3
Uniprot ACC: P40344
Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Up-stream | LIR Motif | Down-stream | Start position | End position | Type | (-2)LIR PSSM score | Exp. Verified (Functional YES/NO) |
---|---|---|---|---|---|---|---|
YRDFLPKNKQ | FLII | RKVPCDKRAE | 68 | 71 | F LIR | 9 | - |
GLAKDMAQER | YYDL | YIAYSTSYRV | 168 | 171 | Y LIR | 6 | - |
NGSPLSPEQM | FEDI | SADYRTKTAT | 202 | 205 | F LIR hfAIM(3) | 10 | - |
EEQELDGVGD | WEDL | QDDIDDSLRV | 270 | 273 | W LIR xLIR hfAIM(3) hfAIM(5) | 22 | YES1 Ref. (4) |
DIDDSLRVDQ | YLIV | FLKFITSVTP | 286 | 289 | Y LIR | 12 | - |
Comments:
1DOI: 10.4161/auto.7.3.14487: "The yeast Atg3 LIR motif (DWEDL) is required for transfer of Atg8 from the Atg8-Atg3 thioester intermediate to PE, but not for the formation of the intermediate. Interestingly, the Atg3 LIR is required for the Cvt pathway, but not for starvation-induced autophagy, and is important for lipidation of Atg8 that is bound to the Cvt pathway cargo receptor Atg19." DOI 10.1074/jbc.M110.113670: "These results clearly indicate that the WEDL sequence of Atg3 HR is a functional AIM and that Atg3 AIM directly interacts with Atg8 in a manner similar to Atg8-Atg19 AIM and LC3-p62 AIM interactions."